How to Mix your HCG Injection Kit

Just received your hCG? Need to know how to mix your hCG from The HCG Institute? Each vial of the hCG hormone for injection comes lyophilized or in a powder form and will need to be mixed with bacteriostatic water before using. Lyophilization improves the storage life of hCG, ensuring that you get your product with its maximum potency.1  

In this article, you’ll learn the step by step procedures on how to reconstitute, measure, and prepare your hCG injections.

A. Gather all supplies needed:

  • hCG vial 
  • bacteriostatic water
  • mixing syringe
  • insulin syringes
  • alcohol prep pads

These supplies will come with the hCG injection kit when you buy from The HCG Institute online.  Make sure that you wash your hands with soap and water and dry well, or use an alcohol-based hand-cleaning product before mixing your hCG injection powder.

B. How to mix your hCG injection powder:

  1. Take off the protective caps of the bacteriostatic water vial and the hCG vial. Tap down the hCG vial to ensure that all powder is collected at the bottom of the vial. Place them on a flat surface.
  2. Open the syringe and needle from their packaging and connect the two, if necessary. Make sure the syringe and the needle are completely tight so that no liquids can leak, even if they came already connected. This also prevents accidental disconnection of the needle.hcg mixing
  3. To draw bacteriostatic water from the vial, pull back on the plunger and pull air into the syringe equal to the amount of liquid you will need to draw. Pull the needle cover straight off the needle insert the needle straight through the rubber stopper.  Push the plunger of the syringe down and inject air into the vial. The bacteriostatic water vials are under vacuum with negative oxygen pressure, rendering it harder to tug the fluid out. Thus, injecting air helps alleviate the vacuum-like pressure pulling against you.
  4. Hold the bacteriostatic water vial upside down and withdraw the amount of bacteriostatic water needed to dilute the hCG to mix hcg
  • 5,000ius     –  Add 5ml of bacteriostatic water into the vial of your hCG.
  • 6,000ius     –  Add 6ml of bacteriostatic water into the vial of your hCG.
  • 11, 000uis  – Add 5.5ml of bacteriostatic water into the hCG vial.
  • 12,000ius   –  Add 12ml of bacteriostatic water into the vial of your hCG.
  1. Clear the air bubbles out of the syringe by tapping it with your finger to get all of the air bubbles to the top. Push up on the plunger slightly until they are all gone. 
  2. When measuring the amount of water, read from the top ring (closest to the needle) and not the bottom ring or the raised section in the middle of the plunger. Make sure the top ring of the rubber seal is at the correct mark. If you are unable to get the correct amount of water transferred, please contact us so that we can adjust your dose to allow for the different concentration.
  3. Insert the diluent into your vial of powdered hCG. Swirl gently until the hCG powder is completely dissolved. Don’t shake.

C. How to draw your hCG medication:

  1. Get your insulin syringe and with the needle cover still on the needle, pull back on the plunger and pull air into hcg mixing
  2. Turn the vial upside down and slowly pull back on the plunger and let the hCG hormone enter the syringe, filling up to the 25 syringe marks. If you received the 11,000IU hCG vial, fill your insulin syringe up to the 12.5 marks. Again, read from the top ring and not the bottom ring. If you are not sure how much hCG you are to inject, please contact us so we can help.
  3. Gently tap the syringe until the air bubbles rise to the top of the syringe barrel. Then push the plunger, forcing the air out of the syringe. Once again pull the plunger back to the prescribed dosage. Repeat these steps if needed.
  4. Prefill all your daily syringes and store them inside the refrigerator. 
  5. Clean the injection site with an alcohol prep pad before administering the hCG injection. Check this link on how to administer hCG injections subcutaneously. 2.
  6. Immediately discard all used syringes in a sharps disposal container to reduce the risk of needle sticks, cuts, or punctures from loose sharps. If you don’t have or did not purchase this together with your medication, you can use a heavy-duty plastic household container as an alternative (an empty liquid laundry detergent container is a good example) that’s able to close with a tight-fitting, puncture-resistant lid, without sharps being able to come out.3.

hcg mixing instructions


1. Manohar P, Ramesh N. Improved lyophilization conditions for long-term storage of bacteriophages. Sci Rep. 2019;9(1):15242. Published 2019 Oct 23. doi:10.1038/s41598-019-51742-4

2. Usach I, Martinez R, Festini T, Peris JE. Subcutaneous Injection of Drugs: Literature Review of Factors Influencing Pain Sensation at the Injection Site. Adv Ther. 2019;36(11):2986-2996. doi:10.1007/s12325-019-01101-6